How does FuGENE 6 work?

FuGENE® 6 Transfection Reagent(a) is a nonliposomal reagent that transfects DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.

What is FuGENE 6?

FuGENE 6 Transfection Reagent is a nonliposomal, multicomponent reagent proven to efficiently transfect more than 700 cell types. Delivery of nucleic acids into cells using liposomal or multicomponent transfection reagents is a well-established method for transfection of mammalian cell lines.

What is FuGENE transfection reagents and how does it work?

FuGENE® HD Transfection Reagent is a multi-component reagent that forms a complex with DNA, then transports the complex into animal or insect cells. It is suitable for use in media with or without serum, and for transient or stable transfection, as well as co-transfections of multiple DNA plasmids.

How does FuGENE work?

FuGENE® HD interacts with nucleic acids to provide efficient and safe entry into the cell, allowing users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and suspension cells. Its simple, easy-to-use protocol allows users to free up valuable lab time.

What is FuGENE HD?

The FuGENE® HD Transfection Reagent is a nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity.

Is Lipofectamine a liposome?

Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. Lipofectamine contains lipid subunits that can form liposomes in an aqueous environment, which entrap the transfection payload, e.g. DNA plasmids.

How does the FuGene 6 transfection reagent protocol work?

FuGENE® 6 Transfection Reagent is a nonliposomal reagent that transfects DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Printed in USA. Revised 4/11.

Can you store FuGene 6 in a glass vial?

Do not freeze or store below 0°C. Formulation and Packaging: FuGENE® 6 Transfection Reagent is a proprietary mixture of lipids and other components in 80% ethanol, ﬁ ltered through a 0.1µm ﬁ lter and supplied in glass vials.

How big is the volume of FuGene 6?

not present 6:1 4:1 3:1 1.5:1 Medium to a ﬁ nal volume of1 100µl 100µl 100µl 100µl DNA amount 2µg 2µg 2µg 2µg Volume of FuGENE® 6 Transfection Reagent 12µl 8µl 6µl 3µl

How much FuGene is needed for dsRNA transfection?

-Always use more FuGENE reagent (µl volume) than RNA (µg mass) -A ratio of 3µl FuGENE to 1-2µg RNA works well in S2 cells -3 uL of FuGENE with 2 uL of dsRNA at a concentration of 1ug/uL is ideal -For co-transfection experiments, if the total amount of RNA exceeds 2µg, increase the amount of FuGENE proportionally.