Common questions How much does a 12 well plate hold?

How much does a 12 well plate hold?

How much does a 12 well plate hold?

Growth Area of Tissue Culture Plates and Dishes

Growth Area (cm2) Maximum Volume (ml)
245 mm (square) 500
6 well 9.5 16.8
12 well 3.8 6.9

What is the surface area of a 24-well plate?

24-Well Plates

Cat.No. Brand Growth Area
83-3526 Corning® 1.9cm2
83-3527 Corning® 1.9cm2
83-3473 Corning® 1.9cm2
83-3738 Corning® 1.9cm2

How many cm2 is a 12 well plate?

3.60 cm2
12 well plate, flat bottom, polystyrene, 3.60 cm2, sterile, 126/cs.

What is the function of well plate?

The well plate has become a standard tool in analytical research and clinical diagnostic testing laboratories¹. ELISA is very common and is the basis of most modern medical diagnostic testing in humans and animals. The wells of the microplate typically hold between tens of nanoliters to several milliliters of liquid.

How much volume does a 48 well plate have?

For 48- and 96-well plates, you can use 300 ul and 100 ul to do the washes, respectively.

How many cells should be seeded in 96 well plate?

I normally suggest not to go for more than 1,00,000 cells per well in a 96 well plate. However, if the incubation time is too long or the cells grow faster, you need to go down further in seeding the cells. You can increase the culture volume to 200 microliters if you want to increase the cell number.

How many cells does it take to seed a 24 well plate?

Useful information for various sizes of cell culture dishes and flasks

Catalog No. Cells at confluency*
6-well 140675 1.2 x 106
12-well 150628 0.5 x 106
24-well 142475 0.24 x 106

How many cm2 is a 24 well plate?

As the growth area in each 24 well plate is 1.9 cm2. So, the total growth surface- area is 1.9*24 = 45.6 cm2.

What is a well plate best used for in the lab?

Industry standard, 96-well plates are designed for all common instruments and can be used for applications such as sample collection, compound preparation, combinatorial chemistry, high throughput screening, nucleic acid purification, bacterial culture growth, and plate replication.

What is the function of microtiter plate?

Microtiter plates are most frequently used for screenings and cultivation of large sample amounts. In order to perform an immunoassay (e. g. ELISA = Enzyme Linked Immuno Sorbent Assay) the bottoms of microtiter plate wells are coated with antibodies to detect specific substances within samples.

Which is the best vessel for Corning cell culture?

This guide gives the recommended medium volumes, approximate growth surface areas and average cell yields for Corning® disposable cell culture vessels including microplates, multiple well plates, Transwell® permeable supports, dishes, flasks, stacked chambers, roller bottles and CellCube® Systems.

How big is the surface area of a Corning well?

**Because these wells are round, the surface area available for cell attachment is dependent on the medium volume used. Corning Multiple Well Plates Single Well Only Well Approx. Total Well Working Diameter Growth Average Volume Volume Plate (Bottom) (mm) Area (cm2) Cell Yield (mL) (mL) 6-well 34.8 9.5 9.5 x 105 16.8 1.9 – 2.9

How big is a Corning cell culture dish?

Insert Format Diameter (mm) Area (cm2) Cell Yield Well Insert 6-well 24 mm 4.67 cm2 4.67 x 105 2.6 1.5 12-well 12 mm 1.12 cm2 1.12 x 105 1.5 0.5 24-well 6.5 mm 0.33 cm2 3.3 x 104 0.6 0.1 96-well 4.26 mm 0.143 cm2 1.4 x 104 0.235 0.075 100 mm dish 75 mm 44 cm2 4.4 x 106 13.0 9.0 Corning Dishes

How big is a 12 mm cell culture vessel?

Insert Format Diameter (mm) Area (cm2) Cell Yield Well Insert 6-well 24 mm 4.67 cm 2 4.67 x 10 5 2.6 1.5 12-well 12 mm 1.12 cm 2 1.12 x 10 5 1.5 0.5