This test detects the presence of the enzyme pyrazinamidase which can be used to distinguish between potentially toxigenic and non-toxigenic Corynebacteria spp. Background. The Elek and guinea pig lethality test have been used to determine whether a Corynebacterium is pathogenic or non-pathogenic.
Does Corynebacterium require isolation?
Infection control issues Follow-up cultures should be done at 48 hours after treatment and again at 2 weeks to document clearance. Isolation should continue until two consecutive cultures 24 hours apart are negative.
Is Ethionamide synthetic drug?
Ethionamide is an antibiotic used to treat tuberculosis. Specifically it is used, along with other antituberculosis medications, to treat active multidrug-resistant tuberculosis. It is no longer recommended for leprosy. It is taken by mouth….Ethionamide.
What are the contraindications of Pyrazinamide?
Who should not take PYRAZINAMIDE?
a type of joint disorder due to excess uric acid in the blood called gout.
severe liver disease.
What kind of neuraminidase does Corynebacterium diphtheriae produce?
Corynebacterium diphtheriae may produce a neuraminidase that cleaves sialic acid from the cell surface into its pyruvate and N-acetylneuraminic acid components. Cord factor (6,6′-di-O-mycoloyl-α,α’-D-trehalose) is a surface component of C diphtheriae, but its role in colonization of the human host is unclear.
Which is the best pathogen in corynebacteria?
The best known pathogen in the group of Corynebacteria is toxigenic C. diphtheriae and C. ulcerans and C. pseudotuberculosis are two other potentially toxigenic species. The three species are able to produce diphtheria toxin—the major virulence factor—and cause serious, potentially fatal disease in humans called diphtheria.
What’s the percentage of Tween 80 for corynebacteria?
For lipophilic Corynebacteria, 0·1–1·0% Tween 80 should be added to the medium (Versalovic 2011 ). Special recommendations for isolation of C. diphtheriae and other potentially toxigenic species ( C. ulcerans and C. pseudotuberculosis) exist.
How to isolate corynebacteria from sheep blood?
Most of the Corynebacterium species can be isolated from a 5% sheep blood agar-based selective medium containing 100 μ g ml −1 of fosfomycin as this group of bacteria is highly resistant to this compound. For lipophilic Corynebacteria, 0·1–1·0% Tween 80 should be added to the medium (Versalovic 2011 ).