What is the restriction enzyme of EcoRI?

What is the restriction enzyme of EcoRI?

EcoRI (pronounced “eco R one”) is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites, and is also a part of the restriction modification system.

Where does the EcoRI enzyme cut?

EcoRI cuts double stranded DNA at the sequence GAATTC, but note that this enzyme, like many others, does not cut in exactly the middle of the restriction sequence (Figure 8.4. 8). The ends of a molecule cut by EcoRI have an overhanging region of single stranded DNA, and so are sometimes called sticky-ends.

Which of the following is the restriction site for EcoRI?

Cut Site: G/AATTC.

What does a restriction enzyme do?

A restriction enzyme is an enzyme isolated from bacteria that cuts DNA molecules at specific sequences. The isolation of these enzymes was critical to the development of recombinant DNA (rDNA) technology and genetic engineering.

What are the reaction conditions for EcoRI restriction enzyme?

Thermo Scientific EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

Can you buy Thermo Scientific EcoRI restriction enzyme online?

ERR#1200 – This item cannot be ordered online. ERR#1200 – This item cannot be ordered online. Order requests are processed and shipped by your local distributor. Learn more Thermo Scientific EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.

Which is the best restriction enzyme from Takara?

DNA Restriction Enzymes from Takara such as EcoRI are high-quality: perform restriction enzyme digestion with reliable restriction endonucleases. Cat. # 1040B contains 5 of Cat. # 1040A.

What does EcoRI stand for in Enzyme cloning?

Restriction Enzyme Cloning Quantity 10,000U Methylation Sensitivity Not dam methylation-sensitive, Not dcm . Enzyme EcoR I Compatible Buffer Unique Buffer (10x Buffer EcoRI ) Sensitive to Heat Inactivation Yes