What test would you do to confirm the identification of Yersinia pseudotuberculosis?

The presence of Y. enterocolitica and Y. pseudotuberculosis in food can be determined quantitatively by a direct culture on selective agar plates. However, confirmatory tests require a combination of cold enrichment, selective enrichment, and subculture on selective agar plates.

Is Yersinia enterocolitica oxidase tested?

Yersinia are a group of Gram-negative, non-spore-forming, oxidase-negative, catalase-positive, lactose-negative, and facultative anaerobic rods (or coccobacilli). Y. enterocolitica is one of three human pathogenic species in the genus Yersinia.

How is Yersinia enterocolitica treatment?

What is the best treatment? Fluoroquinolones are the drugs of choice for Y. enterocolitica infections, based on clinical observations and in vitro antimicrobial resistance studies. Third generation cephalosporins, trimethoprim-sulfamethoxazole and aminoglycosides also appear to be clinically effective.

Is Yersinia enterocolitica indole positive?

Yersinia enterocolitica, indole-negative and indole-positive biotypes. Isolation, identification and sensitivity to chemotherapeutics.

Is Yersinia serious?

Yersinia enterocolitica: A rare but important food safety concern for young children and immune-compromised individuals. Yersinia enterocolitica causes foodborne illness in this country and has the most serious public health significance.

What are the symptoms of Yersinia enterocolitica?

Common symptoms in children are fever, abdominal pain, and diarrhea, which is often bloody. Symptoms typically develop 4 to 7 days after exposure and may last 1 to 3 weeks or longer. In older children and adults, right-sided abdominal pain and fever may be the predominant symptoms and may be confused with appendicitis.

What is the incubation period for Yersinia enterocolitica?

Incubation period is typically 4–6 days (range, 1–14 days). Symptoms include fever, abdominal pain (may mimic appendicitis), and diarrhea (may be bloody and can persist for several weeks). Necrotizing enterocolitis has been described in infants.

What can you eat with Yersinia enterocolitica?

enterocolitica grows well at low temperatures and can withstand freezing and survive in frozen foods for extended periods. Thus, Y. enterocolitica can grow easily at refrigerated temperatures in vacuum-packed meat, boiled eggs, boiled fish, pasteurized liquid eggs, pasteurized whole milk, cottage cheese, and tofu.

Which is the best test for confirming Yersinia enterocolitica?

• Confirmatory laboratory evidence: Isolation of Yersinia enterocolitica orYersinia pseudotuberculosisfrom a clinical specimen . • Supportive laboratory evidence: Detection of Yersinia enterocolitica orYersinia pseudotuberculosisfrom a clinical specimen using a culture-independent diagnostic test (CIDT), such as a PCR.

How is the diagnosis of Yersinia disease made?

Diagnosis is made by isolating the organism from stool, blood, bile, wound, throat swab, mesenteric lymph node, cerebrospinal fluid, or peritoneal fluid. If Yersinia infection is suspected, the clinical laboratory should be notified and instructed to culture on cefsulodin-irgasan-novobiocin (CIN) or other specific for growing it.

What are the symptoms of Yersinia enterocolitica in children?

Yersinia, Culture – Yersinia enterocolitica causes a variety of symptoms depending upon the age of the patient. In children, symptoms often include fever, abdominal pain, and bloody diarrhea.

Where is the best place to test for Yersinia?

Many laboratories do not routinely test for Yersinia, so it is important to notify laboratory personnel when yersiniosis is suspected so that special tests can be done. The organism can also be recovered from other sites, including the throat, lymph nodes, joint fluid, urine, bile, and blood.